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Sequence of primers designed for LAMP amplification of the F1L gene of ORFV
| Primer name | Sequence (5′-3′) | Amplicon size (F2–B2c) |
|---|---|---|
| F3 |
GGTGGTCTACCCCGAGTAC |
782–961a |
| B3 |
GGCGATGAACCACAGCAG |
|
| FIP (F1c + F2) |
TAGATGGCGCCGGGCCAGA-AAACGCGGCTCAGCGG |
|
| BIP (B1c + B2) | CTCCTTCATGGGGCTCTTCGAC-GCAGCAGCAGCACGAG |
aNucleotide position based on the sequence of F1L gene region of ORFV isolate Jilin-Nongan (GenBank accession no. JQ271535.1).
