Table 2.
Project | Primary primers | Secondary primers | Number of specimens | Success rate (%) | Number of specimens >200 bp with <2% Ns |
---|---|---|---|---|---|
CHUBL |
LCO1490_t1/HCO2198_t1 |
None |
94 |
87% |
78 |
SAOST |
LCO1490_t1/HCO2198_t1 |
LCO1490_t1/MLepR1; MLepF1/HCO2198_t1a |
95 |
83% |
79 |
COCSA |
LCO1490_t1/HCO2198_t1 |
CrustDF1/CrustDR1 |
190 |
65% |
124 |
OZFWZb |
C_LepFolF/C_LepFolR |
ZplankF1_t1/ZplankR1_t1 |
90 |
70% |
63 |
OZFWC – Plates 1 and 2b |
ZplankF1_t1/ZplankR1_t1 |
C_LepFolF/C_LepFolR |
70 |
34% |
24 |
OZFWC – Plates 3 and 4b |
LCO1490_t1/HCO2198_t1 |
C_LepFolF/C_LepFolR |
49 |
37% |
18 |
OZFWC – Plates 5-9b |
C_LepFolF/C_LepFolR |
ZplankF1_t1/ZplankR1_t1 |
164 |
68% |
112 |
Combined | 752 | 66.2% | 498 |
Primer sequences and references are provided in Additional file 1: Appendix A. The primer pairs used for both PCR amplification and cycle sequencing for each individual specimen are available through BOLD.
a This primer combination involving mini-primers uses two separate PCR reactions to attempt to amplify the full-length barcode region in two fragments.
b These samples were run on 96-well plates including mixed microcrustaceans, and thus the sample sizes do not reflect full plates.