Figure 5.

Allogeneic mixed T cell proliferation assays. imDCs, mDCs or PsL-EGFmAb-treated mDCs were co-cultured with freshly isolated human CD4⁺, CD4⁺CD25⁺ and CD4⁺CD25^– T cells, respectively, then proliferation assays were performed and the results are shown as fold increases in [³H]TdR incorporation. Flow cytometric analysis was also performed to detect CD4⁺CD25⁺Foxp3⁺ Tregs. A, DCs co-cultured with CD4⁺ T cells. B, DCs co-cultured with CD4⁺CD25⁺ T cells. C, DCs co-cultured with CD4⁺CD25^– T cells. D, A suppression assay was performed to evaluate the suppressive function of CD4⁺CD25⁺ T cells using the same method. Tregs were either co-cultured with PsL-EGFmAb-treated mDCs (T1) or freshly isolated from health adults. The mean ± SD of four independent experiments is shown. ^#p < 0.05, ^##p < 0.01.