(A) LRN assayed using a short-duration training paradigm. LRN PI scores increase progressively as a function of training duration. Mg2+ block mutations do not inhibit LRN. Rather, elav/dNR1(N631Q) flies have slightly improved LRN compared to other transgenic control flies at training durations of 10 and 20 s. *p < 0.05 and **p < 0.01, n = 8–12 for all data.
(B) Expression of a dNR1(N631Q) transgene restores normal memory in dNR1EP331 mutants. **p < 0.01, n = 6–8 for all data.
(C) Acute feeding of 1 mM RU486, starting one day prior to training, significantly impairs LTM formation in elavGS/dNR1(N631Q) flies. **p < 0.001, n = 8–12 for all data.
(D) Expression of a dNR1(N631Q) transgene in the ellipsoid body of the central complex, Feb170/dNR1(N631Q) and C42/dNR1(N631Q), and in the MBs, OK107/dNR1(N631Q) and C309/dNR1(N631Q), significantly impairs LTM formation. **p < 0.001, n = 10 for all data.
See also Figure S4.