Figure 6.

Noggin increases stem/progenitor cell proliferation. Noggin was overexpressed under the control of the NSE promoter. A–E, BrdU labeling and immunofluorescence reveals an increase in multiple precursor populations: GFAP⁺ putative stem cells, PSA-NCAM⁺ migratory precursors, and GFAP⁻PSA-NCAM⁻ putative transient amplifying progenitors. F, Although all three precursor populations are expanded, the proportion of GFAP⁺ putative stem cells is increased at the expense of other precursors. Arrows point to GFAP⁺BrdU⁺ cells, and arrowheads point to PSA-NCAM⁺BrdU⁺ cells. NCAM, PSA-NCAM. Error bars represent mean ± SEM of three experiments. *p < 0.05; **p ≤ 0.01 by unpaired Student's t test.