Table 1.
Primers used for detection of virulence genes in uropathogenic Escherichia coli
| Gene | Primer name | Primer sequence (5'-3') | Size of product (bp) | Reference |
|---|---|---|---|---|
|
set-1 |
set-1-F |
GTGAACCTGCTGCCGATATC |
147 |
[21] |
| |
set-1-R |
ATTTGTGGATAAAAATGACG |
|
|
|
sen |
Sen-F |
ATGTGCCTGCTATTATTTAT |
799 |
[21] |
| |
Sen-R |
CATAATAATAAGCGGTCAGC |
|
|
|
astA |
astA-F |
ATGCCATCAACACAGTATAT |
110 |
[21] |
| |
astA-R |
GCGAGTGACGGCTTTGTAGT |
|
|
|
sigA |
sigA-F |
TCCTCGGTATTATTTTATCC |
408 |
[21] |
| |
sigA-R |
CGTAACCCCTGTTGTTTCCAC |
|
|
|
sap |
Sap_f |
TACCCTCCACAACAGAGAATG |
832 |
[21] |
| |
Sap-R |
TACCCTCCACAACAGAGAATG |
|
|
|
pic |
Pic-F |
ACTGGATCTTAAGGCTCAGGAT |
500 |
[21] |
| |
Pic-R |
GACTTAATGTCACTGTTCAGCG |
|
|
|
pap |
pap3 |
GCAACAGCAACGCTGGTTGCATCAT |
336 |
[27] |
| |
pap4 |
AGAGAGAGCCACTCTTATACGGACA |
|
|
|
cnf1 |
cnf1 |
AAGATGGAGTTTCCTATGCAGGAG |
498 |
[27] |
| |
cnf2 |
TGGAGTTTCCTATGCAGGAG |
|
|
|
hlyA |
hly1 |
AACAAGGATAAGCACTGTTCTGGCT |
1177 |
[27] |
| |
hly2 |
ACCATATAAGCGGTCATTCCCGTCA |
|
|
|
sfa |
sfa1 |
CTCCGGAGAACTGGGTGCATCTTAC |
410 |
[28] |
| |
sfa2 |
CGGAGGAGTAATTACAAACCTGGCA |
|
|
|
afa |
afa1 |
GCTGGGCAGCAAACTGATAACTCTC |
750 |
[28] |
| |
afa2 |
CATCAAGCTGTTTGTTCGTCCGCCG |
|
|
|
iuc |
iuc1 |
ATGAGAATCATTATTGACATAATTG |
1482 |
[29] |
| |
iuc2 |
CTCACGGGTGAAAATATTTT |
|
|
|
fim |
fim1 |
GAGAAGAGGTTTGATTTAACTTATTG |
559 |
[30] |
| |
fim2 |
AGAGCCGCTGTAGAACTGAGG |
|
|
|
papGI |
papGJ96-F |
TCGTGCTGAGGTCCGGAATTT |
461 |
[31] |
| |
papGJ96-R |
TGGCATCCCCCAACATTATCG |
|
|
|
papGII |
papGIA2-F |
GGGATGAGCGGGCCTTTGAT |
190 |
[31] |
| |
papGIA2-R |
CGGGCCCCCAAGTAACTCG |
|
|
|
papGIII |
prsJ96-F |
GGCCTGCAATGGATTTACCTGG |
258 |
[31] |
| |
prsJ96-R |
CCACCAAATGACCATGCCAGAC |
|
|
|
kpsMT |
kpsM481F |
CCATCGATACGATCATTGCACG |
400 |
[32] |
| |
kpsM481R |
ATTGCAAGGTAGTTCAGACTCA |
|
|
|
iha |
IHA-F |
CTGGCGGAGGCTCTGAGATCA |
827 |
[23] |
| |
IHA-R |
TCCTTAAGCTCCCGCGGCTGA |
|
|
|
iron |
IRONEC-F |
AAGTCAAAGCAGGGGTTGCCCG |
665 |
[23] |
| |
IRONEC-R |
GACGCCGACATTAAGACGCAG |
|
|
|
ompT |
ompT-F |
ATCTAGCCGAAGAAGGAGGC |
559 |
[33] |
| |
ompT-R |
CCCGGGTCATAGTGTTCATC |
|
|
|
usp |
usp -F |
ACATTCACGGCAAGCCTCAG |
440 |
[22] |
| |
usp -R |
AGCGAGTTCCTGGTGAAAGC |
|
|
|
iss |
Iss-F |
ATCACATAGGATTCTGCCG |
309 |
[34] |
| |
Iss-R |
CAGCGGAGTATAGATGCCA |
|
|
|
irp2 |
Irp2-F |
AAGGATTCGCTGTTACCGGAC |
413 |
[34] |
| |
Irp2-R |
AACTCCTGATACAGGTGGC |
|
|
|
tsh |
Tsh-F |
ACTATTCTCTGCAGGAAGTC |
824 |
[34] |
| |
Tsh-R |
CTTCCGATGTTCTGAACGT |
|
|
|
vat |
Vat-F |
TCCTGGGACATAATGGTCAG |
981 |
[34] |
| |
Vat-R |
GTGTCAGAACGGAATTGT |
|
|
|
cva |
Cva-F |
TGGTAGAATGTGCCAGAGCAAG |
1181 |
[34] |
| Cva-R | GAGCTGTTTGTAGCGAAGCC |