Table 2.
Primers used for detection of uropathogenic Escherichia coli serogroups[35]
| Serogroup | Target gene | Primer name | Primer sequence (5’-3’) | Size of product (bp) |
|---|---|---|---|---|
|
O1 |
wzx |
wl-14632 |
GTGAGCAAAAGTGAAATAAGGAACG |
1098 |
| |
|
wl-14633 |
CGCTGATACGAATACCATCCTAC |
|
|
O6 |
wzy |
wl-14646 |
GGATGACGATGTGATTTTGGCTAAC |
783 |
| |
|
wl-14647 |
TCTGGGTTTGCTGTGTATGAGGC |
|
|
O7 |
wzx |
wl-14648 |
CTATCAAAATACCTCTGCTGGAATC |
610 |
| |
|
wl-14649 |
TGGCTTCGAGATTAAACCTATTCCT |
|
|
O8 |
orf469 |
wl-14652 |
CCAGAGGCATAATCAGAAATAACAG |
448 |
| |
|
wl-14653 |
GCAGAGTTAGTCAACAAAAGGTCAG |
|
|
O16 |
wzx |
wl-14654 |
GGTTTCAATCTCACAGCAACTCAG |
302 |
| |
|
wl-14655 |
GTTAGAGGGATAATAGCCAAGCGG |
|
|
O21 |
wzx |
wl-14676 |
CTGCTGATGTCGCTATTATTGCTG |
209 |
| |
|
wl-14677 |
TGAAAAAAAGGGAAACAGAAGAGCC |
|
|
O75 |
wzy |
wl-17413 |
GAGATATACATGGGGAGGTAGGCT |
511 |
| |
|
wl-17414 |
ACCCGATAATCATATTCTTCCCAAC |
|
|
O2 |
wzy |
wl-14636 |
AGTGAGTTACTTTTTAGCGATGGAC |
770 |
| |
|
wl-14637 |
AGTTTAGTATGCCCCTGACTTTGAA |
|
|
O4 |
wzx |
wl-14642 |
TTGTTGCGATAATGTGCATGTTCC |
664 |
| |
|
wl-14643 |
AATAATTTGCTATACCCACACCCTC |
|
|
O15 |
wzy |
wl-14672 |
TCTTGTTAGAGTCATTGGTGTATCG |
183 |
| |
|
wl-14673 |
ATAAAACGAGCAAGCACCACACC |
|
|
O18 |
wzx |
wl-14656 |
GTTCGGTGGTTGGATTACAGTTAG |
551 |
| |
|
wl-14657 |
CTACTATCATCCTCACTGACCACG |
|
|
O22 |
wzx |
wl-14660 |
TTCATTGTCGCCACTACTTTCCG |
468 |
| |
|
wl-14661 |
GAAACAGCCCATGACATTACTACG |
|
|
O25 |
wzy |
wl-14666 |
AGAGATCCGTCTTTTATTTGTTCGC |
230 |
| |
|
wl-14667 |
GTTCTGGATACCTAACGCAATACCC |
|
|
O83 |
wzx |
wl-14668 |
GTACACCAGGCAAACCTCGAAAG |
362 |
| |
|
wl-14669 |
TTCTGTAAGCTAATGAATAGGCACC |
|
|
E. coli |
16SrRNA |
wl-3110 |
AGAGTTTGATCMTGGCTCAG |
919 |
| wl-3111 | CCGTCAATTCATTTGAGTTT |