Table 3.
Primers used for detection of antimicrobial resistant genes in uropathogenic Escherichia coli
| Antimicrobial agent | Resistance gene | Sequence | Size (bp) | References |
|---|---|---|---|---|
|
Streptomycin |
aadA1 |
(F) TATCCAGCTAAGCGCGAACT |
447 |
[36] |
| |
|
(R) ATTTGCCGACTACCTTGGTC |
|
|
|
Gentamicin |
aac(3)-IV |
(F) CTTCAGGATGGCAAGTTGGT |
286 |
[36] |
| |
|
(R) TCATCTCGTTCTCCGCTCAT |
|
|
|
Sulfonamide |
sul1 |
(F) TTCGGCATTCTGAATCTCAC |
822 |
[36] |
| |
|
(R) ATGATCTAACCCTCGGTCTC |
|
|
|
Beta-lactams |
blaSHV |
(F) TCGCCTGTGTATTATCTCCC |
768 |
[36] |
| |
|
(R) CGCAGATAAATCACCACAATG |
|
|
|
Beta-lactams |
CITM |
(F) TGGCCAGAACTGACAGGCAAA |
462 |
[36] |
| |
|
(R) TTTCTCCTGAACGTGGCTGGC |
|
|
|
Chloramphenicol |
cat1 |
(F) AGTTGCTCAATGTACCTATAACC |
547 |
[36] |
| |
|
(R) TTGTAATTCATTAAGCATTCTGCC |
|
|
|
Chloramphenicol |
cmlA |
(F) CCGCCACGGTGTTGTTGTTATC |
698 |
[36] |
| |
|
(R) CACCTTGCCTGCCCATCATTAG |
|
|
|
Tetracycline |
tet(A) |
(F) GGTTCACTCGAACGACGTCA |
577 |
[37] |
| |
|
(R) CTGTCCGACAAGTTGCATGA |
|
|
|
Tetracycline |
tet(B) |
(F) CCTCAGCTTCTCAACGCGTG |
634 |
[37] |
| |
|
(R) GCACCTTGCTGATGACTCTT |
|
|
|
Trimethoprim |
dfrA1 |
(F) GGAGTGCCAAAGGTGAACAGC |
367 |
[38] |
| |
|
(R) GAGGCGAAGTCTTGGGTAAAAAC |
|
|
|
Quinolones |
qnr |
(F) GGGTATGGATATTATTGATAAAG |
670 |
[39] |
| (R) CTAATCCGGCAGCACTATTTA |