Table 1.

Technical evaluation of the BGM competitive ELISA

Technical validation step	BGM ELISA
Target	MMP-9 and -12 degradation of biglycan
Lower limit of detection (LDL)	1.54 ng/mL
Dilution recovery of human serum	100% (1:2), 103% (1:4)
Dilution recovery of human plasma	97% (1:2), 99% (1:4)
Dilution recovery of rat serum	107% (1:2), 102% (1:4)
Dilution recovery of rat plasma	89% (1:2)
Dilution recovery of mouse serum	105% (1:2)
Dilution recovery of mouse plasma	97% (1:2)
Intra-assay variation	10%
Inter-assay variation	15%

Percentage dilution recovery and percentage intra/inter variation are indicated as mean values of different samples diluted 1:2 and 1:4 and as mean variation between ten individual determinations of human samples, respectively.