Table 7.
RNAi of RNA-seq and ChIP-seq candidates
| AQR position (%) (n ≥ 100; p ≤ 0.05 compared to mab-5(e1751) | ||||||
|---|---|---|---|---|---|---|
|
Genotype |
1 |
2 |
3 |
4 |
5 |
|
|
wild-type |
100 |
0 |
0 |
0 |
0 |
|
|
mab-5(e1751) |
0 |
0 |
0 |
8 |
92 |
|
|
ceh-20(RNAi) |
89 |
7 |
1 |
3 |
0 |
|
|
mab-5(e1751); ceh-20(RNAi) |
0 |
0 |
6 |
35 |
59 |
|
|
RNAi in a mab-5( e1751 ) background |
|
|||||
|
Up in mab-5( gof ) |
|
|
|
|
|
Description |
|
Secreted/transmembrane |
|
|
|
|
|
|
|
C37C3.6/mig-63 |
0 |
0 |
2 |
24 |
74 |
Papilin protease inhibitor |
|
F16C3.2 |
0 |
0 |
1 |
21 |
74 |
Secreted |
|
K02G10.7/aqp-8 |
0 |
0 |
0 |
26 |
74 |
aqp-8 aqueporin |
|
F17E9.11/lys-10 |
0 |
0 |
0 |
23 |
77 |
Lysosomal protein |
|
C35D10.11 |
0 |
0 |
0 |
22 |
78 |
Transmembrane DUF2012 |
|
C02A12.4/lys-7 |
0 |
0 |
1 |
20 |
79 |
Lysosomal protein |
|
F11C7.5/osm-11 |
0 |
0 |
0 |
21 |
79 |
Secreted |
|
F10E7.4/spon-1 |
0 |
0 |
0 |
20 |
80 |
F-spondin |
|
C37A5.4/fipr-23 |
0 |
0 |
1 |
16 |
83 |
Fungal-induced, secreted |
|
Gene expression |
|
|
|
|
|
|
|
C08C3.2/bath-15 |
0 |
0 |
0 |
28 |
67 |
BTB-MATH |
|
C08C3.3/mab-52 |
0 |
0 |
1 |
22 |
77 |
Hox |
|
F26D12.1/fkh-73 |
0 |
0 |
1 |
18 |
81 |
Forkhead |
|
Cytoplasmic signaling/cytoskeleton |
|
|
|
|
|
|
|
K08F8.11,2 |
0 |
0 |
1 |
24 |
75 |
MAPKAP3-like |
|
T04H1.9/tbb-6 |
0 |
0 |
0 |
21 |
76 |
Beta tubulin |
|
T07C4.9/nex-2 |
0 |
0 |
0 |
23 |
77 |
Annexin |
|
Y17G7B.14/arrd-8 |
0 |
0 |
0 |
20 |
80 |
Arrestin |
|
F59A1.9 |
0 |
0 |
0 |
20 |
80 |
F-box containing |
|
M60.7 |
0 |
0 |
1 |
17 |
82 |
Ankyrin-repeat containing |
|
Metabolism |
|
|
|
|
|
|
|
C53A3.21 |
0 |
0 |
0 |
25 |
75 |
Nitrophenylphosphatase |
|
Down in mab-5( lof ) |
|
|
|
|
|
|
|
ZC443.6/ugt-16 |
0 |
0 |
0 |
22 |
78 |
UDP Glucuronosyltransferase |
|
W10G11.1 |
0 |
0 |
1 |
18 |
81 |
Secreted cys-rich DUF19 |
|
F48C1.8 |
0 |
0 |
0 |
19 |
81 |
Secreted, conserved |
|
ChIP seq targets |
|
|
|
|
|
|
|
T23G4.2 |
0 |
0 |
0 |
38 |
62 |
Unsimilar cytoplasmic |
|
T24D1.4/tag-179 |
0 |
0 |
0 |
33 |
67 |
Transmembrane glycosyltransferase ALG10 |
|
C55B7.7/gly-2 |
0 |
0 |
0 |
33 |
67 |
Secreted glycosyltransferase, family 18 |
|
T23G4.1/tlp-1 |
0 |
0 |
0 |
30 |
70 |
Zinc-finger transcription factor |
|
F22D6.2 |
0 |
0 |
0 |
29 |
71 |
AN1-like zinc finger |
|
F54F2.2/zfp-1 |
0 |
0 |
0 |
27 |
73 |
Zinc finger PHD-like |
|
F32E10.1/nol-10 |
0 |
0 |
0 |
26 |
74 |
Nucleolar protein |
|
M05B5.4 |
0 |
0 |
1 |
23 |
76 |
Phospholipase A2-like |
|
R13A5.5/ceh-13 |
0 |
0 |
0 |
24 |
76 |
Hox-B1/Labial |
|
W09G12.8 |
0 |
0 |
0 |
24 |
76 |
Peptide N-glycanase/PAW domain |
|
F33G12.6 |
0 |
0 |
0 |
24 |
76 |
Protein phosphatase 2C-like |
|
R01E6.3/cah-4 |
0 |
0 |
0 |
24 |
78 |
Carbonic anhydrase |
|
B0361.2 |
0 |
0 |
0 |
21 |
79 |
CwfJ C-terminal domains |
|
F42C5.9 |
0 |
0 |
0 |
20 |
80 |
Actin-like PH domain |
|
F21A10.2 |
0 |
0 |
0 |
20 |
81 |
Transmembrane myelin regulatory factor |
|
DY3.2/lmn-1 |
0 |
0 |
0 |
19 |
81 |
Nuclear lamin |
| C09H6.3/mau-2 | 0 | 0 | 0 | 19 | 81 | Chromatid cohesion factor |
1Also DOWN in mab-5(lof).
2Also a MAB-5 top 50 ChIP seq target.
3Also a MAB-5 ChIP seq target.
4AQR position as described in Methods and Figure 2D.