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. 2013 Apr 23;110(19):7946–7951. doi: 10.1073/pnas.1220205110

Fig. 5.

Fig. 5.

SA receptors are not required for SA effect on endocytosis. (A–D) Immunolocalization of PIN2 in WT (A and B), and npr1-1 (C and D) treated with 25 μM BFA (A and C) or cotreated with 50 μM SA for 90 min after a 30-min pretreatment with 50 µM SA (B and D). (E) Quantification of the effect of SA on the BFA-induced PIN2 internalization in A–D (nWT;DMSO = 513 BFA bodies from 26 roots; nnpr1-1;DMSO = 169 BFA bodies from 10 roots; nWT;SA = 68 BFA bodies from 34 roots; nnpr1-1;SA = 85 BFA bodies from 33 roots). (F–I) Immunolocalization of PIN1 in WT (F and G), and npr1,3,4 (H and I) treated with 25 μM BFA (F and H) or cotreated with 50 μM SA for 90 min after a 30-min pretreatment with 50 µM SA (G and I). (J) Quantification of the effect of SA on the BFA-induced PIN1 internalization in F–I (nWT;DMSO = 120 BFA bodies from eight roots; nnpr1,3,4;DMSO = 115 BFA bodies from eight roots; nWT;SA = 114 BFA bodies from 13 roots; nnpr1,3,4;SA = 65 BFA bodies from four roots). Values in E and J represent the mean surface area relative to the control. Data are means ± SD; **P < 0.01 (Student’s t test). (Scale bars: 10 μm).