Fig. 5.
Effects of NCOR1ΔID on TRα1PV-repressed lipogenic genes in the epididymal fat of Thra1PV/+ mice (A–C). The expression of mRNA was determined by real-time RT/PCR as described in Materials and Methods. The data are expressed as mean ± SEM (n = 5–7) and P values are indicated. (D) Lack of recruitment of NCOR1ΔID to the promoter of the C/ebpα gene abolishes the dominant negative effect of TRα1PV. ChIP assay was carried out using normal rabbit IgG (lanes 2, 4, 6, and 8) or anti-NCOR1 antibody (PHQQ) (lanes 1, 3, 5, and 7) as described in Materials and Methods. Data are expressed as mean ± SEM (n = 3). NS, Not significant.