Fig. 1.

Use of elongated protein fibrils to tether Dam1 to beads. (A) Schematic of an end-on kinetochore–MT connection with a Dam1 ring tethered to the kinetochore central hub. (B) Lateral attachment of a Dam1-coated bead to an MT in vitro (roughly to scale). Soluble Dam1 subunits are used to promote completion of the full Dam1 ring. (C) Chemical steps involved in Dam1 conjugation to the bead using the 100-nm-long CC tether or directly to the surface with a heterobifunctional cross-linker and anti-GFP IgG.