Fig. 7.
Infusing CNS fluid with rapoE particles increases ISF Aβ levels. (A) A 38-kDa cutoff microdialysis probe with a specialized side infusion port was implanted in hippocampi of young PDAPP/TRE mice. After a stable 6-h baseline, 1 μg of freshly prepared rapoE2 or rapoE4 (in artificial CSF solution) was infused at a flow rate of 0.07 μL/min directly at the site of microdialysis in PDAPP/E2 or PDAPP/E4 mice, respectively, and ISF [eAβ1-x] was monitored for an additional 6 h. Shown is the relative change in [eAβ1-x] within each mouse after rapoE2 or rapoE4 infusion compared with its mean baseline period. (B) The mean percent change in [eAβ1-x] following rapoE infusion relative to the baseline period was calculated for each mouse in each group. Differences between baseline and treatment periods were assessed for each group using paired t tests. n = 4–7 mice per group; **P < 0.01.