Fig. 2.

E4orf4 induces caspase activation in a subpopulation of cells in the Drosophila eye and wing discs. Eye (A–F) and wing (G–R) discs from third-instar larvae (A–L: grown at 29 °C; M–R: grown at 24 °C) with the indicated genotypes [GMR>lacZ (A–C), GMR>E4orf4 (D–F), en>GFP (G–I), en>E4orf4 (J–L), ms1096>lacZ (M–O), and ms1096>E4orf4 (P–R)] were stained with antibodies to β-galactosidase (A, M) or E4orf4 (D, J, P) (Left) or to activated caspase-3 (B, E, H, K, N, Q) (Center). (Right) (C, F, I, L, O, R) Merge of the two channels. Representative images are shown.