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Mutagenesis: in vitro DNA-binding and in vivo complementation experiments. Under each mutation the outcomes of two experiments are listed. DNA binding measured by band shift is highlighted blue and complementation is highlighted pale. +, positive; −, no binding detected and no complementation. Downward arrows indicate weaker complementation based on heterocyst frequency. WT HetR complementation gave ∼8.5% heterocysts at 48 h. Blank fields mean no results.
