Fig. 2.

Water-conducting states for Glt_ph (EAAT) of the SLC1 family (Upper) and GlpT from the MFS superfamily (Lower). (Left) Topology of the protein using the crystal structure, highlighting in different colors functionally relevant domains and repeats. Though Glt_ph is simulated as a trimer, here only the monomer in the intermediate state is depicted, with the transport and trimerization domains colored in green and purple, respectively. (Center) Representative water-conducting frame from the simulations. (Right) Time series for the number of water permeation events along the efflux and influx directions, radius of the narrowest part along of the aqueous lumen, and C_α rmsd of the TM region. The vertical black dashed lines represent the snapshots shown in the molecular images, and the horizontal red dashed lines show the minimal radius to accommodate a water molecule.