Fig. 3.

LuxD is an essential component of the artificial alkane biosynthetic (CEDDEC) pathway. (A) GC-MS TIC of hydrocarbons extracted from E. coli cells expressing CEDDEC in ΔfadD BL21* (DE3) in the presence of 100 μg⋅mL⁻¹ 13-methyl tetradecanoic acid. (B) GC-MS TIC of hydrocarbons extracted from WT E. coli cells expressing CE-DEC (luxC, luxE, and NpAD with the luxD gene removed from the operon; Fig. S1). The absence of pentadecane is indicated by the gray arrow. All cells were grown and induced as described in Experimental Procedures.