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Identification of alkane biosynthesis limitations in the E. coli host. Total alkane, aldehyde (C14 and C16), and fatty alcohols (C14 and C16) extracted from control cells [BL21* (DE3)], cells expressing the cyanobacterial pathway (NpAR/NpAD), the artificial CEDDEC pathway, and the artificial CEDDEC pathway supplemented with tetradecanoic acid (+ FA). n = 3 biological replicates; error bars represent SE of the mean. Cells were grown and induced as detailed in Experimental Procedures.
