Fig. 2.

RPA on the ssDNA loop stimulates PCNA loading on the DL. (A) PCNA loading was performed on the DL (lanes 3 and 4) and its derivative that had an additional oligonucleotide covering the ssDNA loop (DL-CAP; lanes 1 and 2). (B) PCNA loading on the C2 (lanes 1–3) and the DL (lanes 4–6) was performed under standard conditions in the presence or absence of RFC (0.08 μM), RPA (0.8 μM), and E. coli SSB protein (0.8 μM) as indicated. (C) Percentages of loaded PCNA were calculated from B with repeated experiments (n = 3–4). Error bars represent the SDs.