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. 2013 Apr 22;110(19):7648–7653. doi: 10.1073/pnas.1220220110

Fig. 2.

Fig. 2.

Tertiary screening results with NDPs. Relative initial velocities of OleD variants with 2-chloro-4-nitrophenyl glucoside (donor 1) as donor and NDPs as acceptors. The relative initial velocity for TDP-16 was set equal to 1 for all NDPs. The label for each variant are the amino acid mutation(s) introduced into the parental gene sequence of oleD[P67T/S132F/A242L/Q268V] (the template for OleD variant TDP-16). The determined kcat(Km)–1 for TDP-16 with donor 1 saturating and the respective NDP varied are listed after each NDP label. SDs of absorbance readings for TDP-16 (n = 2) typically varied by <5% for each data point over the course of each assay.