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. 2013 Apr 22;110(19):7784–7789. doi: 10.1073/pnas.1305859110

Fig. 2.

Fig. 2.

SSRP1 depletion inhibits SHM. Cells treated with either anti-SSRP1#1 siRNA or MedGC were subjected to sequencing analysis. (A) Results of the mutation analysis for HygGFP and the V(D)J region. P values for the significance of reduction by SSRP1 knockdown are shown in each graph (Fisher’s exact test). The detailed results are shown in Fig. S3. (B) Transcript levels of JP8Bdel-ER, HygGFP, and Igh were unaffected by SSRP1 knockdown. Total RNAs purified 48 h after transfection were analyzed by qRT-PCR, and the transcript levels relative to the MedGC were calculated. Data shown are the mean and SD from three independent experiments. (C) Cell-cycle distribution was unaltered by SSRP1 knockdown during AID activation. (Left) Cell-cycle histograms of representative samples. (Right) Fractions of cells in the G1, S, and G2/M of triplicated samples. Values are the mean and SD.