Fig. 5.
Relationship between kinetics of binding and potency at inhibiting the bioactivity of GM-CSF. (A) Shown are the equilibrium dissociation constants and rate constants for the rhGM-CSF and mAb complexes and comparison with their IC50 values from the TF-1 proliferation assay. (B) A graphical representation of the data from A analyzing the correlation between neutralization activity on the y axis vs. the KD (Left), kd (Center), and ka (Right) on the y axis. A nonparametric Spearman correlation test was used to calculate the P values reported for each comparison. ka, P = 0.4131; kd, P = 0.0436; KD, (P = 0.0315).