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. 2013 Apr 22;110(19):7720–7725. doi: 10.1073/pnas.1213431110

Fig. 3.

Fig. 3.

MMEJ is used frequently to repair DSBs in mammalian cells. (A) EGFP-MMEJ/HR-MluI competition repair substrate. (B) U2OS cells with single integration of EGFP-MMEJ/HR-MluI substrate were induced with I-SceI and sorted for EGFP-positive cells, and PCR and digestion analysis was performed with percentages of digestible products shown. (C) U2OS (from B) and BJ and HME1 cell populations carrying EGFP-MMEJ/HR-MluI were assayed as in B, with repair frequencies of three independent experiments. (D and E) EGFP-HR-MluI repair substrate and assay.