Figure 4. Under CR conditions, the atg32Δ-dependent mutational block of macromitophagy alters the age-related chronology of changes in vital mitochondrial functions.

WT and atg32Δ strains were cultured in the nutrient-rich YP medium initially containing 0.2% glucose. Effect of theatg36Δ mutation on the rate of oxygen consumption by cells (A), electrochemical potential across the inner mitochondrial membrane IMM (ΔΨ) (B), level of cytochrome c in purified mitochondria (M) and the cytosolic fraction (C) (C), and cellular level of ATP (D). Data in A, B and D are presented as means ± SEM (n = 6-9).