Figure 5. Sir2 overexpression in the fat body did not affect fat storage and fat cell morphology.

Relative body fat content as quantified by coupled colorimetric assay (A) and body weight (B) of male and female UAS-Sir2>S₁106-Gal4 flies feeding on medium with or without 400 μM RU486 showed no significant differences. Diameters of fat droplets were measured in fat bodies of UAS-Sir2>S₁106-Gal4 L3 larvae after Bodipy staining (C) and no differences were observed. Bodipy staining furthermore reveals no differences in size and structure of fat cells as well as droplet size in L3 larvae (D, G), adult females (E, H) or males (F, I) after feeding on ethanol (top row) or RU486 (bottom row). A-C, Data are represented as means + SEM. Mann-Whitney-U-Test was performed using GraphPad Prism 6.00, n = 4 for coupled colorimetric assay and body weight, n = 7 for fat droplet diameter measurements. D-I, scale bar represents 50 μm.