Fig. 2.

Sex differentiation in hepatic Cyp2e1 expression pattern. A: Cyp2e1 mRNA, protein and activity level in cyclic females at E in ovariectomized (OV) and male mice. Comparisons took place with basal Cyp2e1 mRNA levels at E. CYP2E1-dependent PNP activity. B: effect of hormonal replacement with 17β-estradiol (E₂) and/or progesterone on Cyp2e1 expression. Comparisons took place with basal Cyp2e1 mRNA levels in OV mice. Values are expressed as means ± SE (n = 10). CYP2E1 Western blot images contain 1 sample per treatment. Each lane in the capture represents CYP2E1 protein amount in the microsomes of 1 mouse. C: Correlation of changes in relative Cyp2e1 mRNA levels with plasma progesterone and E₂ levels. **P < 0.01, ***P < 0.001.