Fig. 6.

Assessment of impact of female steroid hormones on PI3K/Akt/FOXO1 signaling pathway. Western blot analysis of Akt and FOXO1 phosphorylation (p) in hepatic nuclear, cytosolic, and total (t) cellular proteins of male mice, cyclic females at E, and OV mice that received placebo or hormonal replacement using pellets containing estradiol and/or progesterone. Western blot images contain 1 sample per treatment, which is representative of 3 samples per group tested. Each lane in the capture represents total or phosphorylated Akt or FOXO1 in total cellular, nuclear, or cytosolic proteins of 1 mouse, respectively.