Fig. 8.

Assessment of impact of female steroid hormones and tamoxifen on GH/STAT5b signaling pathway. Western blot analysis of STAT5b phosphorylation in hepatic nuclear and cytosolic proteins of male mice, cyclic females at E, treated with normal saline or tamoxifen, and OV mice that received placebo or hormonal replacement using pellets containing estradiol and/or progesterone. Western blot images contain 1 sample per treatment, which is representative of 3 samples per group tested. Each lane represents total or phosphorylated STAT5b in nuclear or cytosolic proteins of 1 mouse.