Assessment of effect of tamoxifen on Cyp2e1 expression and PI3K/Akt/FOXO1 activation. A: effect of tamoxifen on Cyp2e1, Hnf-1α, β-catenin, and Pgc-1α mRNA expression. Values are expressed as means ± SE (n = 10). **P < 0.01, ***P < 0.001. B: assessment of tamoxifen-induced effect on Akt, FOXO1, and JNK activation by Western blot. Western blot images contain 1 sample per treatment, which is representative of 3 samples per group tested. Each lane in the capture represents total or phosphorylated Akt, FOXO1, or JNK in total cellular proteins of 1 mouse.