Table 2.

Effect of ANG-(1–7) and/or A779, BAPTA, and/or thapsigargin in maximum cytosolic calcium concentration of isolated proximal tubules from rats

Experimental Groups	[Ca2+]i, nM	n
Control	101 ± 2	6
ANG-(1–7) (10−12 M)	252 ± 8*	5
ANG-(1–7) (10−9 M)	203 ± 3*	6
ANG-(1–7) (10−6 M)	153 ± 8*	5
A779 (10−6 M)	126 ± 2*	5
ANG-(1–7) (10−12 M) + A779	114 ± 7†	5
ANG-(1–7) (10−9 M) + A779	96 ± 4†	5
ANG-(1–7) (10−6 M) + A779	105 ± 5†	5
BAPTA (5 × 10−5 M)	58 ± 3*	5
BAPTA + ANG-(1–7) (10−9 M)	106 ± 3†	5
BAPTA + ANG-(1–7) (10−6 M)	82 ± 2*†	6
Thapsigargin (10−5 M)	341 ± 9*	5
Thapsigargin + ANG-(1–7) (10−9 M)	356 ± 9*†	5
Thapsigargin + ANG-(1–7) (10−6 M)	342 ± 12*†	5

Values are the means ± SE; n = number of tubules (each tubule corresponds to the average of 10 areas). [Ca²⁺]_i, intracellular Ca²⁺ concentration.

^*P < 0.01 vs. control;

^†P < 0.01 vs. ANG-(1–7) alone in the respective dose.