Fig. 7.

AQP11 protein expression in kidneys from 20-day-old mice. A and B: glomeruli with emerging AQP11 positive convoluted PT (S1 segment) from urinary pole of Bowman's capsule (arrow; magnification: ×400). C: kidney sections from AQP11 knockout (KO) mice at the same age served as a negative control and showed no immunostaining in kidney tissue (magnification: ×400). D: WT kidney section stained with no primary antibody served as a second negative control (magnification: ×400). E: severely deformed AQP11-positive tubules in the Aqp11^sjdssjds kidney. F: in WT kidney strong AQP11 immunolabeling is seen in the cortex (CX) and outer stripe of outer medulla (O/M). I/M, inner stripe of outer medulla (n = 5–6 per group; magnification: ×100). Scale bar = 300 μm. Smaller size of mutant kidney section (E) reflects reduced size of kidney in homozygous Aqp11^sjds/sjds mutants when compared with kidney from WT littermate (F) and confirms our previously published data (28).