Fig. 8.

Glucose-induced AQP11 expression. A: real-time quantitative RT-PCR analysis of glucose-induced Aqp11 gene expression in PT cells (PTC) cultured overnight in glucose-free medium supplemented with or without 3–25 mM glucose. Results are expressed as means ± SE, *P < 0.005 vs. untreated cells. All samples were in triplicate and mRNA samples were isolated from each well. B: representative RT-PCR analysis (n = 4) revealed a similar glucose-induced Aqp11 gene expression (516-bp PCR product) in PTC (samples were normalized to total mRNA). Cells were cultured in glucose-free DMEM supplemented with 1% FBS and with or without 3–25 mM glucose overnight.