Fig. 6.

Bmal1 and CLOCK transactivate the human Scn5a promoter reporter. A: lumicycle data showing the circadian expression pattern of the hScn5a promoter reporter construct transiently transfected in mouse C2C12 (skeletal muscle) cell line; n = 3. B: luciferase assay results of transfection experiments using either the Per1 reporter gene or the hScn5a reporter gene in mouse fibroblast NIH/3T3 cells (n = 6–10/condition). CLOCK (C) and BMAL1 (B) significantly transactivate Per1 and hScn5a reporter genes (black bars) relative to control transfections (white bars). Activation of the hScn5a reporter is significantly diminished when Bmal1R91A was overexpressed with CLOCK (gray bar) or ClockΔ19 was overexpressed with BMAL1 (vertical striped bar). Values are expressed as means ± SE. *P < 0.05 compared with the hScn5a reporter gene; #P < 0.05 compared with hScn5a reporter gene + CLOCK + BMAL.