Fig. 1.

Vesicular nucleotide transporter (VNUT) is expressed in Calu-3 cells. A: RT-PCR analysis of VNUT expression using primers common to VNUT-1 and VNUT-2 mRNAs (predicted amplification size 296 bp; left) or specific for VNUT-1 (V-1, 209 bp) or VNUT-2 (V-2, 181 bp) (right); see Table 1 for primer sequences. B: Calu-3 cell lysate proteins were electrophoresed on 4–10% SDS-PAGE and transferred and analyzed by Western blotting with anti-VNUT antibody (V) or its antigen-preabsorbed antiserum (PA). C: Coomassie blue (left) and Western (right) blots of extracts from uninduced (a) and induced Escherichia coli (b) and partially purified human VNUT (hVNUT; c); samples were electrophoresed on 10% SDS-PAGE. Numbers at left indicate size in bp (A) or kDa (B and C).