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VNUT short hairpin RNA2 [shRNA2 (sh2)] and shRNA6 (sh6) reduced VNUT expression in Calu-3 cells. Cells were infected with lentivirus carrying scramble shRNA (scr), VNUT shRNA2, or VNUT shRNA6 and selected for 10 days with puromycin before RNA and protein extraction. A: quantification of VNUT mRNA levels using primers for the common region for both VNUT isoforms (see Table 1). B: representative Western blot. TUB, tubulin. Values are means ± SD from 3 independent experiments. *P < 0.05.
