Fig. 7.

VNUT knockdown reduces nucleotide content in mucin granules. Calu-3 cells were infected with lentiviruses bearing scramble or VNUT shRNA6 and studied at 3 days postinfection. A and B: mRNA and proteins were quantified in the whole cell extract. C and D: mucin granules (MG) were isolated, and their content was measured as described in Fig. 3 legend. Values are means ± SD from 3 independent experiments. *P < 0.05. Insets: representative Western blots for VNUT in whole cells (B) and mucin granules (C) from scramble- and VNUT shRNA-infected cells.