Fig. 5.

Ki67p-GFP expression in the developing nervous system. The developing cerebral cortex of an embryonic mouse was electroporated in utero with a plasmid containing the 1.5-kb proximal promoter of the human Ki67 gene upstream of GFP (Ki67p-GFP) (122) and a ubiquitously expressed reporter, CAGGp-RFP (90), at embryonic day 14.5 and fixed after 24 h. The ventricular (VZ) and subventricular zones (SVZ) contain neural precursors. These precursors produce postmitotic neurons that will migrate through the intermediate zone (IZ) to the cortical plate (CP) via the radial glial fibers projecting to the pial surface. Arrows indicate presumptive postmitotic neurons (red arrows) and mitotically active precursors (yellow arrows).