Table 1.
DNA-labeling dyes and their characteristics
| Name of Stain | Cell Permeable | Excitation Maximum, nm | Emission maximum, nm |
|---|---|---|---|
| Propidium iodide | No | 536 | 617 |
| 7-AAD | No | 488 | 655 |
| DAPI | Yes | 350 | 470 |
| Draq5 | Yes | 488–647 | 665 |
| Hoescht 33258 | Yes | 352 | 461 |
| Hoescht 33342 | Yes | 350 | 461 |
| Hoescht 34580 | Yes | 392 | 440 |
| Vybrant DyeCycle Violet | Yes | 405 | 437 |
| Vybrant DyeCycle Green | Yes | 488 | 534 |
| Vybrant DyeCycle Orange | Yes | 488, 532 | 563 |
| Vybrant DyeCycle Ruby | Yes | 488, 633/5 | 686 |
7-AAD, 7-amino-actinomycin D; DAPI, 4′,6′-diamidino-2-phenylindole.