Fig. 1.

Urinary ANG-(1–7) and ANG II in angiotensin-converting enzyme type 2 (ACE2) wild-type (WT) and ACE2 knockout (KO) mice. Twenty-four-hour urine samples were collected in the presence of 20 μl of 6 N HCl, mixed with protease inhibitor cocktail, subjected to solid-phase extraction, and analyzed by radioimmunoassay. Peptide levels were normalized to excretion per day. Values are means ± SE of 8–9 mice per group. On 2-way ANOVA, there were no differences among the groups.