Figure 4.

Semi-quantitative RT-PCR (A) and qPCR (B–G) analyses of the expression of the IL-4 receptor subunits in human gingival fibroblasts. The RT-PCR analysis was performed in cells isolated from three different individuals (A). The numbers on the right of the images represent the number of cycles in the PCR reaction. In B–D is shown the time-dependent effect of IL-1β (100 pg·mL⁻¹) on the mRNA expression of IL4R, IL13RA1 and IL13RA2. In E–G is shown the effect by IL-4 (30 ng·mL⁻¹) on IL-1β induced regulation of IL4R, IL13RA1 and IL13RA2. The values represent means ± SEM for four wells per group. Samples were analysed in duplicates and normalized with RPL13A. The statistically significant effects are shown by asterisks (*, P < 0.05; **, P < 0.01; ***, P < 0.001).