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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: FEBS J. 2013 Feb 28;280(10):2285–2293. doi: 10.1111/febs.12165

Table 2.

Protein Binding Constants Derived from SPR Analysis.

GAGs Protein
Interaction		 kon (1/MS) koff (1/S) KD (M)
HS SHH 1.5 × 104 0.017 1.2 × 10−6
FGF2 7.4 × 107 0.53 7.2 × 10−9
FGF1 2.2 × 105 0.11 4.9 × 10−7
Commercial KS SHH 437 0.022 5.0 × 10−5
FGF2 3.0 × 105 0.29 9.7 × 10−7
FGF1 N.B. N.B. N.B.
Isolated KS SHH 344 0.022 6.5 × 10−5
FGF2 6.2 × 105 0.46 7.4 × 10−7
FGF N.B. N.B. N.B.

N.B., no binding detected.