Figure 2. Long-term multi-lineage expression of retrovirally encoded proteins.

(A) 4 weeks after reconstitution with bone marrow transduced with VSV-I-A^b (left column) or mock transduced (right column), B10.MBR mice were bled, and peripheral blood mononuclear cells (PBMC) were examined by cell surface staining with the lineage markers CD4, CD8, B220, CD11b and CD11c. Cells were then analyzed by flow cytometry. Shown is one representative of three independent experiments. (B) 11 weeks mice were reconstituted with bone marrow transduced with VSV-I-A^b, PBMC were harvested and stimulated with LPS. After 72 hours, cells were stained with antibodies specific for IA^b, IA^k, B220 and CD3 prior to analysis by flow cytometry. Shown are B220+ cells (upper panels) gated on transduced GFP+ cells (solid black line) or GFP- cells (solid gray line. Cells from naïve B10.MBR stimulated with LPS were used as a control (dashed line). Also shown are GFP+ (black line) and GFP- (gray line) CD3+ T cells (lower panel). (C) 24 weeks after bone marrow reconstitution, recipients of VSV-I-A^b (left panel) or mock (right panel) transduced bone marrow were bled, and GFP and IA^b expression in lymphocytes was analyzed by flow cytometry. Shown is one representative of three independent experiments.