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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Gene Ther. 2012 Jul 26;20(5):478–486. doi: 10.1038/gt.2012.57

Figure 5. Expression of retrovirally encoded MHC class II prevents T cell cytokine production in response to MHC class II mismatched splenocytes.

Figure 5

B10.MBR mice were reconstituted with VSV-I-Ab (white bars) or control VSV-GFP (black bars) transduced bone marrow. 8-10 weeks after reconstitution, mice were immunized with 107 irradiated B10.QBR splenocytes and sacrificed 10 days later. (A) Splenocytes were re-stimulated with irradiated B10.QBR splenocytes and analyzed by ELISPOT for IL-2, IL-4 or IFN-γ. Data are presented as spots per 106 T cells for each cytokine examined. Shown are the cumulative mean values and standard deviations obtained from three separate experiments. (B) Splenocytes were re-stimulated with B10.QBR or syngeneic B10.MBR splenocytes, and after 48 hours, supernatants were collected and analyzed for cytokine expression by Luminex assay. Background response to B10.MBR has been subtracted from the results shown. Shown are the cumulative mean values and standard deviations obtained from three separate experiments.