Skip to main content
. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Mol Cancer Ther. 2013 Feb 26;12(5):696–704. doi: 10.1158/1535-7163.MCT-12-0868

Figure 3. Combined treatment of PF02341066 and radiation induces more apoptosis in H3122 cells than either agent alone.

Figure 3

H3122 and H460 cells were treated with 6 Gy radiation. Western blots at 0, 0.25, 0.5, 1, 2, and 4 hours after radiation treatment using labeled antibodies to phospho- and total ALK, phospho- and total STAT3, phospho- and total Akt, phospho- and total ERK are shown for H3122 cells (3A) in DMSO and (3B) in 0.4 µM PF02341066. H3122 (3C) and H460 (3D) cells were treated with 0, 0.4, and 1 µM PF02341066 for 2 hours followed by radiation treatment with 0 or 6 Gy. Expression of total caspase-3, and cleaved caspase-3 was determined by immunoblotting 48 h post radiation treatment. For all experiments, actin was probed to demonstrate equal loading.