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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: FEBS J. 2013 Apr 2;280(10):10.1111/febs.12231. doi: 10.1111/febs.12231

Figure 3.

Figure 3

Summary of CS saccharides ion abundances and total abundance from leukocytes from four donors. CS total ion abundance was calculated from the sum of 3 Δ4,5-unsaturated disaccharides, 3 saturated disaccharides and GalNAcSO3 and was normalized per 10 million cells. Triplicate samples of extracted GAGs were digested using chondroitinase ABC for donors 1(A), 2(B) and 4(D) for each population except for B cells for which duplicates were acquired. Duplicates of extracted GAGs were digested using chondroitinase ABC for donor 3(C) for each cell population. CS total ion abundances in counts (E) and CS total saccharides quantities in pmols (F) summarized from four donors. Box plot (E) shows that the median (range) for CS total ion abundances in counts was 1.06*107 (4.69*106–2.58*107), 1.56*107 (1.06*107 –3.91*107), 2.99*106 (1.51*106–8.14*106), 1.97*106 (3.80*105–2.49*106), 5.79*106 (2.47*106–2.14*107) and 1.26*107(7.19*106 –7.75*107) for the PMN, PBMC, T, B, NK and monocyte, respectively. Box plot (F) shows that the median (range) for CS total saccharides quantities in pmols was 2898.65 (733.94–4410.74), 2953.09 (1495.21–5517.81), 330.63(208.66–1307.76), 276.69(67.37–354.40), 817.80(354.10–3425.85) and 2838.23(1041.45–10890.79) for the PMN, PBMC, T, B, NK, and monocyte, respectively. Box-whisker plot notation was the same as shown in the Fig. 2 legend.