Fig. 3.

HIF1A and CD34 expression in differentiating human mast cells. CD133+ cells were obtained from PBMC of individual blood donors by magnetic separation and were cultured under mast cell differentiating conditions for up to five weeks. Aliquots of PBMC (PBMC), purified CD133+ (CD133+), and cell cultured for indicated time were lysed and the lysates were used for RNA purification. The RNA obtained from the different cell populations was used to determine HIF1A expression by real-time RT-PCR. The data obtained from a total of 35 donors and 33 mast cell cultures, are presented as median, lower and upper quartile, and minimum and maximum for each experimental condition from at least 11 cultures. * indicates significant differences at p < 0.05 as compared to the CD133+ cells