Fig. 2.

Time courses of blood glucose levels (a) and the development of mechanical allodynia (b) and thermal hyperalgesia (c) in normoglycemic mice and in STZ-treated animals. Statistical analysis of the results was conducted using two-way repeated measures ANOVA, followed by Bonferroni's multiple comparison. Blood glucose concentration: drug effect: F[1,42] = 392.57; p < 0.0001; time effect: F[3,42] = 25.3; p < 0.0001; interaction: F[3,42] = 26.7; p < 0.0001. ***p < 0.001 compared to nondiabetic mice. Mechanical allodynia was evaluated using von Frey test and thermal hyperalgesia was assessed by means of hot plate test (56 °C). Mechanical nociceptive thresholds: drug effect: F[1,42] = 96.33; p < 0.0001; time effect: F[3,42] = 28.64; p < 0.0001; interaction: F[3,42] = 29.41; p < 0.0001. Thermal nociceptive thresholds: drug effect: F[1,42] = 80.51; p < 0.0001; time effect: F[3,42] = 10.68 p < 0.0001; interaction: F[3,42] = 17.84; p < 0.0001. Significance: **p < 0.01; ***p < 0.001 compared to normoglycemic mice