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. 2013 Apr 20;49(5):346–353. doi: 10.1007/s11626-013-9599-z

Figure 1.

Figure 1.

Preparation of a rabbit anti-imp13 antibody. (A) SDS–PAGE of GST-tagged imp13 proteins. Lane 1 whole cell lysates before IPTG induction. Lane 2 whole cell lysates after IPTG induction. Lane 3 the supernatant of cell lysates after IPTG induction. Lane 4 purified GST-tagged imp13 using Glutathione Sepharose 4B beads. (B) Western blot analysis for the retained GST antibody in the anti-sera. GST-tagged imp13 and GST proteins were separated by 10% SDS–PAGE and proteins were transferred to a nitrocellulose membrane. Lanes 1–4 blots were probed with anti-serum prepared primary antibody (1:2,000), followed by goat-anti rabbit IgG/HRP secondary antibody. And retained GST antibody was detected in the anti-serum before treatment (lanes 1 and 2). No GST antibody was detected in the anti-sera after treatment with GST beads (lanes 3 and 4). Lanes 5–6 blots were probed with anti-GST primary antibody (1:1,000) as a control.