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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Biomaterials. 2012 Oct 22;34(2):10.1016/j.biomaterials.2012.09.055. doi: 10.1016/j.biomaterials.2012.09.055

Figure 2.

Figure 2

(A) Porcine dermis (PD) was treated with reactive catechol-functionalized microbeads (MB-CAT) at physiologic pH (pH 7.4), followed by overnight incubation in PBS on shaker. (B) PD samples were treated with a mild reducing agent (glutathione – 20 mM.) to increase the availability of sulfhydryl groups for covalent attachment with maleimide functionalized microbeads (MB-MAL). Subsequently, MB-MAL were incubated for 30 mins followed by overnight incubation in PBS on a shaker.